Life Science Buffers
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 250G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 250G
Research Products International Corp PIPES Sodium Salt 500 G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4, Low Endotoxin) - 100ml
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration and tested for endotoxin.
Teknova 1M Tris-HCl, pH 7.5. 500ml Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 5X TBE DNA Sequencing Grade Solution. 4L, Non-Sterile. 4 pack.
445mM Tris, 445mM Boric Acid, 10mM Ethylenediaminetetraacetic acid (EDTA)
Apexbio Technology LLC Annexin V-HF647/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds double-stranded DNA producing blue fluorescence and normally cannot penetrate intact cell membranes However during late apoptosis or in dead cells DAPI can pass through compromised membranes to stain the nucleus Concurrent staining with Annexin V-HF647 and DAPI allows differentiation of cells at different stages of apoptosis and identification of dead cells
Apexbio Technology LLC One-step TUNEL Cy5 Apoptosis Detection Kit 20rxns
One-step TUNEL Cy5 Apoptosis Detection Kit is designed for detecting cell nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases are activated cleaving genomic DNA between nucleosomes and resulting in DNA fragmentation into segments approximately 180-200 bp or multiples thereof The fragmented genomic DNA contains exposed 3 -OH ends which are labeled with fluorescent-tagged dUTP in a TdT (Terminal Deoxynucleotidyl Transferase) catalyzed reaction allowing visualization and detection by fluorescence microscopy or flow cytometry (Ex/Em 649 nm/670 nm) This kit has a broad range of applications and can be utilized to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells
eMolecules Oakwood Chemicals Manganese powder ?325 mesh ?99% trace metals basis 5g 480149922 099246 7439-96-5 MFCD00011111 54.938 Mn
Oakwood Chemicals Manganese powder ?325 mesh ?99% trace metals basis 5g 480149922 099246 7439-96-5 MFCD00011111 54.938 Mn
Apexbio Technology LLC HyperScript RT SuperMix for qPCR (with gDNA wiper) 50 rxns
HyperScript RT SuperMix for qPCR is a reverse transcription reaction premix based on HyperScript Reverse Transcriptase a novel enzyme engineered from M-MLV (RNase H-) Reverse Transcriptase featuring significantly reduced RNase H activity and greatly enhanced thermo-stability Due to its improved heat tolerance HyperScript Reverse Transcriptase is especially suitable for reverse transcription of RNA templates with complex secondary structures HyperScript RT SuperMix for qPCR (with gDNA wiper) is specifically designed for two-step qRT-PCR assays The 5 SuperMix contains all necessary components for reverse transcription Simply adding template RNA and RNase-Free ddH2O allows rapid reaction initiation The included 4 gDNA wiper efficiently eliminates potential genomic DNA contamination from samples rapidly and thoroughly prior to reverse transcription The kit is particularly suitable for reverse transcription reactions from RNA templates of low concentration
ILEX LIFE SCIENCES LLC TRYPSIN P10-021100 100ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (488) 100 tests
EdU Flow Cytometry Assay Kits (488) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The unique biological groups on EdU and 6-FAM Azide allow fluorescent labeling of proliferating cell DNA after conjugation enabling imaging with low background and high detection sensitivity The EdU Flow Cytometry Assay Kits (488) use 6-FAM azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (EXC/EMI 496/516 nm)) with green fluorescence