Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC Tapso | 68399-81-5 | 98.0% | 100 G
TAPSO is a widely used biological buffer and pH stabilization reagent. It offers an effective pH range between 7 and 8, with pKa values typically falling between 7.5 and 9.0. This compound contains Tris groups and has been observed to exhibit reactive activity with zwitterionic glycine peptides. It is primarily intended for research applications.
- Effective pH range of 7-8.
- pKa values ranging from 7.5-9.0.
- Contains Tris groups.
- Shows reactive activity with zwitterionic glycine peptides.
- Suitable for various research applications.
Ethos Biosciences Dilurex PBS Casein Sucrose Blocker, 1000ml, Ethos
Dilurex PBS Casein Sucrose Blocking Buffer offers the best price to performance ratio on the market. The base system is phosphate buffered saline - perfect for systems that are using PBS as a diluent or reaction buffer. Casein is used as the blocking protein which binds to unoccupied hydrophobic or charged sites on assay surfaces preventing unwanted non-specific binding of antibodies, enzymes, or other proteins leading to clearer, more reliable signals. Sucrose stabilizes your mixture by preserving the folding integrity of proteins during drying or freezing. Choose one of our other blocking buffers if your assay is detecting phosphoproteins, uses biotin/streptavidin detection, Alkaline Phosphatase detection, autoimmune assays, or aldehyde coated substrates in case of potential interference.
eMolecules 7365-45-9 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | 7365-45-9 | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820
eMolecules 2167889-81-6 | tert-butyl 4-(hydroxymethyl)-2,2-dimethyl-piperidine-1-carboxylate | MFCD30497054 | 1g
Medchem Express | NSC-70220 | 5mg | 495799510 | HY-101796 | 4551-00-2 | 325.367 | C22H15NO2
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool<|a>
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
DULBECCOS Dulbeccos Phosphate Buffer
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Research Products International Corp PBS, 10X Powder Concentrate, 10 L
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
Sigma Aldrich Fine Chemicals Biosciences Iscoves Modified Dulbeccos
ISCOVES Iscoves Modified Dulbeccos
Sigma Aldrich Fine Chemicals Biosciences TRIS Glycine buffer soluti1L
TRIS Glycine buffer SOLUTION1L
eMolecules 174346-82-8 | tert-Butyl 2-(hydroxymethyl)azetidine-1-carboxylate | MFCD03790936 | 1g
Ambeed | 3-Chlorothiobenzamide | 5g | 600842207 | A516790 | 2548-79-0 | MFCD00276993 | 171.640 | C7H6ClNS
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool<|a>
STEMCELL Technologies RoboSep™ Buffer 2, Size: 250 mL
RoboSep™ Buffer 2 is recommended for select EasySep™ cell separation protocols. Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.