Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-1000.010.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 1 Kilogram
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-5000.0.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set Crystals, 250 g
Trizma Pre-set Crystals, 250 g.
Sigma Aldrich Fine Chemicals Biosciences Trizma hydrochloride, 1185-53-1, MFCD00012590, 500 g
Linear Formula: NH2C(CH2OH)3 · HCl, Molecular Weight: 157.60, BioPerformance Certified, cell culture tested, ≥99.0% (titration), Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride.
Boston Bioproducts Inc MES Buffer (1 M, pH 6.5) - 500ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
Sigma Aldrich Fine Chemicals Biosciences HEPES anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 7365-45-9 | MFCD00006158 | 1KG
HEPES anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 1KG
INNOVAPREP LLC ELUTION FLUID CAN - PBS
FluidPrep Elution Fluid Can - PBS0.075% Tween 20/PBS. Recommended for classical analytical methods.1 can.Each can provides up to 30 extractions.
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Research Products International Corp TE Buffer 20X Solution pH 8.0, 250 Milliliters
Formulation:
EDTA: 20 mM
Research Products International Corp TRIS BUFFERED SALINE, 10X SOLUTION, 10 LITER
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 1 Liter
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%